sqhC [2019-08-05 09:11:07]

squalene-hopene cyclase, biosynthesis of sporulenes, protection of the spore against oxidative stress

locus: BSU_19320

pI: 7.63

mw: 71.05 kDa

protein length: 632 aa

gene length: 1899 bp

function: biosynthesis of sporulenes

product: squalene-hopene cyclase

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 2,102,168 2,104,066

The protein

Catalyzed reaction/ biological activity

• Squalene = hop-22(29)-ene (according to Swiss-Prot), production of sporulenes, polycyclic terpenoid lipids [Pubmed|18436644]
• protection of the spore against oxidative stress [Pubmed|18436644]

Protein family

• terpene cyclase/mutase family (single member, according to UniProt)

Structure

• [PDB|2SQC] (from Alicyclobacillus acidocaldarius, 32% identity) [pubmed|9931258]

[SW|Localization]

• surrounds the forespores [Pubmed|18436644]

Expression and Regulation

Operons

• genes: [gene|24EB23F594F2B0EEA2E055857BA14FD9E361E0FD|sqhC]-[gene|E0645DD4A7A871457300139A7E5986EF11C07387|sodF]
  description: [Pubmed|18436644]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190,18436644], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulation

  • expressed during sporulation in the mother cell [Pubmed|18436644]

Biological materials

Mutant

• BKE19320 ([gene|24EB23F594F2B0EEA2E055857BA14FD9E361E0FD|sqhC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACGCTCAACTCCTTCA, downstream forward: _UP4_GATTCTATTGAAAAGGAGAC
• BKK19320 ([gene|24EB23F594F2B0EEA2E055857BA14FD9E361E0FD|sqhC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACGCTCAACTCCTTCA, downstream forward: _UP4_GATTCTATTGAAAAGGAGAC

References

• 18436644,9931258